Genebank: Genomics-based disruption of wheat genetic resources for plant breeding

Funding

Federal Ministry of Education and Research (BMBF)

Duration

2016 – 2022

Research partners

Leibniz Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Julius Kühn Institut (JKI)

Industrial partners

Gesellschaft für Erwerb und Verwertung von

Schutzrechten (GVS) mbH; KWS Lochow GmbH; Limagrain GmbH

Team

F. Longin

Dhe aim of the project is to open up the wheat collection of the IPK Gatersleben for breeding through an integrated approach of genomics, phenomics, biodiversity informatics and precision pre-breeding. We will fingerprint ~22,000 accessions of the IPK genebank using the latest marker technology. These will form the basis for the development of four innovative and complementary strategies to identify new useful alleles or gametes:

 

1. the 22,000 accessions will be screened for resistance to yellow rust, brown rust and fusarium. Both phenotypic and genotypic data will be analysed using a new algorithm that allows the construction of a non-stratified population for association mapping (GWAS). This population will be sequenced using RenSeq technology to detect genes and alleles through haplotype-based GWAS. 2.

2. In the search for new traits, we will focus on genetic variation that is important for open wheat flowering and thus for hybrid wheat breeding. We will use genomics-based selection and backcrossing to identify key genes responsible for open pollination.

3. combine molecular physiology and population genomics methods to perform targeted allele mining for candidate genes involved in nitrogen use efficiency.

4. we will use genomic selection tools in pre-breeding to unravel genetic variation for grain yield. The four strategies will be embedded in biodiversity informatics activities to analyse the extensive data with new tools in population genomics and quantitative genetics.